Fig. 4. ICV injection of αBgt decreases hypothalamic neuropeptides involved in food intake. Male mice were fasted for 24 h and refed for 24 h. αBgt (2 µg/mouse) was administered by ICV injection; after 6 h of refeeding, the hypothalamus was removed. (n refed=3, αBgt refed =4) (A) POMC mRNA, (B) AgRp mRNA, (C) NPY mRNA. Cumulative food intake is shown at (D) 4 h, (E) 8 h and (F) 24 h after refeeding (saline=5, n= αBgt =4). For gene expression analysis, ACTB was used as the endogenous control. Student's t-test analysis was used to test differences between groups. * p <0.05.